Development of an in situ biosurfactant production technology for enhanced oil recovery Final Report

......................................................................................................................................... 16 Overview......................................................................................................................................... 16 Materials and Methods ................................................................................................................ 18 Results............................................................................................................................................. 25 Discussion and Conclusions......................................................................................................... 56 4. Molecular methodologies to detect and characterize biosurfactant producers and their use to determine the prevalence of biosurfactant producers in oil reservoirs.......59 Abstract .......................................................................................................................................... 59......................................................................................................................................... 59 Introduction................................................................................................................................... 59 Materials and Methods ................................................................................................................ 60 Results............................................................................................................................................. 65 Discussion....................................................................................................................................... 82 5. Characterization of Bacillus licheniformis strain RS-1 and related strains for suitability in MEOR bioaugmentation in diverse oil formations ..................................85 Abstract .......................................................................................................................................... 85......................................................................................................................................... 85 Introduction................................................................................................................................... 85 Materials and Methods ................................................................................................................ 85 Results............................................................................................................................................. 88 Discussion....................................................................................................................................... 96 6. Microbial Stimulation Treatment of High Water-Cut Wells in the Viola Formation, Pontotoc County, OK....................................................................................................98 Abstract .......................................................................................................................................... 98......................................................................................................................................... 98 Introduction................................................................................................................................... 98 Methodology .................................................................................................................................. 99 Results........................................................................................................................................... 102 Economic analysis ....................................................................................................................... 106